Subtract Images

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Calculates the difference image between two images. The subtrahend image can optional be scaled by a factor read from a text file (img_out = img1 - c * img2). Every intensity value in the difference image below zero is set to zero!

This plugin subtracts one sequence of images from another sequence. For subtracting one background image from a sequence use the dpdak Image Options.

Parameter

  • Out Directory: Path to a directory where the difference images are saved.
  • Correction File (optional): Path to a text file with one column of numbers. The column should have the same size as the image sequence (one correction factor per image).

Inputs

  • ImagePath1: The path to the minuend image.
  • ImagePath2: The path to the subtrahend image.

Outputs

  • SubImage: The path to the difference image.